1BTL
CRYSTAL STRUCTURE OF ESCHERICHIA COLI TEM1 BETA-LACTAMASE AT 1.8 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.100, 64.400, 91.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.800 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.660 |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Number of reflections | 22510 * |
| Completeness [%] | 94.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 * | 6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 10 (%sat) | |
| 3 | 1 | drop | phosphate | 60 (mM) | |
| 4 | 1 | reservoir | phosphate | 60 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 46 (%) | |
| 6 | 1 | reservoir | acetone | 4 (%(v/v)) |
