1BTI
CREVICE-FORMING MUTANTS IN THE RIGID CORE OF BOVINE PANCREATIC TRYPSIN INHIBITOR: CRYSTAL STRUCTURES OF F22A, Y23A, N43G, AND F45A
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.120, 22.210, 28.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.059 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9.7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein solution | 8 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 0.3-0.4 (M) | |
| 3 | 1 | reservoir | potassium phosphate | 1.5 (M) |
