1BTC

THREE-DIMENSIONAL STRUCTURE OF SOYBEAN BETA-AMYLASE DETERMINED AT 3.0 ANGSTROMS RESOLUTION: PRELIMINARY CHAIN TRACING OF THE COMPLEX WITH ALPHA-CYCLODEXTRIN

Experimental procedure

Spacegroup name	P 31 2 1
Unit cell lengths	86.100, 86.100, 144.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	9.000 - 2.000
R-factor	0.177
Rwork	0.177
RMSD bond length	0.014
RMSD bond angle	3.080
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.037 *
Total number of observations	20136 *
Number of reflections	15187 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	5.4 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	2	acetate	0.1 (M)
2	1	2	ammonium sulphate	50 (%sat)
3	1	2		0.1 (M)
4	1	2	2-mercaptoethanol	18 (mM)
5	1	2	EDTA	1 (mM)