1BT6

P11 (S100A10), LIGAND OF ANNEXIN II IN COMPLEX WITH ANNEXIN II N-TERMINUS

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	280
Detector technology	IMAGE PLATE
Collection date	1998-04-25
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	80.600, 56.400, 64.300
Unit cell angles	90.00, 114.50, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.233
Rwork	0.228
R-free	0.30700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a4p
RMSD bond length	0.019
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.060	0.610 *
Total number of observations	29481 *
Number of reflections	9774
<I/σ(I)>	20.6	1.96
Completeness [%]	96.8
Redundancy	6.25	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1	Tris-HCl	50 (mM)
3	1	1	dithiothreitol	1 (mM)
4	1	2	PEG4000	10 (%)
5	1	2	2-propanol	10 (%)
6	1	2	Tris-HCl	100 (mM)