1BT4
PHOSPHOSERINE AMINOTRANSFERASE FROM BACILLUS CIRCULANS SUBSP. ALKALOPHILUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.323, 92.876, 45.499 |
| Unit cell angles | 90.00, 106.67, 90.00 |
Refinement procedure
| Resolution | 29.300 - 2.300 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bjn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.135 | 0.219 |
| Number of reflections | 16466 | |
| <I/σ(I)> | 7.3 | 5 |
| Completeness [%] | 99.4 | 96.4 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | macroseeding | 4.6 | CRYSTALLIZED AT ROOM TEMPERATURE FROM 0.1 M SODIUM ACETATE BUFFER, PH 4.6, 2-6% GLYCEROL, 2-4% PEG 20000, USING MACROSEEDING TECHNIQUES, macroseeding |
