1BSY

STRUCTURAL BASIS OF THE TANFORD TRANSITION OF BOVINE BETA-LACTOGLOBULIN FROM CRYSTAL STRUCTURES AT THREE PH VALUES; PH 7.1

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-05-08
Detector	RIGAKU
Spacegroup name	P 32 2 1
Unit cell lengths	53.960, 53.960, 112.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.240
R-factor	0.2335
Rwork	0.234
R-free	0.27900
RMSD bond length	0.008 *
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.280
High resolution limit [Å]	2.240	2.240
Rmerge	0.038	0.540
Total number of observations	283429 *
Number of reflections	9293
<I/σ(I)>	24.1	2
Completeness [%]	96.4	95.6
Redundancy	3.07	3.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.1		pH 7.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-30 (mg/ml)
2	1	reservoir	HEPES	0.01 (M)
3	1	reservoir	ammonium sulfate	2.2-2.8 (M)