1BSW

ACUTOLYSIN A FROM SNAKE VENOM OF AGKISTRODON ACUTUS AT PH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OTHER
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	63.170, 63.170, 95.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.950
R-factor	0.171
Rwork	0.171
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iag
RMSD bond length	0.013
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.100
High resolution limit [Å]	1.950	1.950
Rmerge	0.083	0.283
Number of reflections	12809
Completeness [%]	88.3	76.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MPD	25.5 (%(v/v))
2	1	drop	protein	10 (mg/ml)
3	1	reservoir	Tris-HCl	0.02 (M)
4	1	reservoir	NaAc-HAc	0.02 (M)
5	1	reservoir	MPD	51 (%(v/v))