1BSM
P.SHERMANII SOD(FE+3) 140K PH8
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 140 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-05 |
| Detector | SIEMENS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.181, 84.319, 107.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.000 - 1.350 |
| R-factor | 0.206 * |
| Rwork | 0.205 |
| R-free | 0.23600 |
| Starting model (for MR) | FE SOD PDB ENTRY 1AR5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 20.900 * |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.160 | 1.390 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.083 * | |
| Number of reflections | 72107 | |
| <I/σ(I)> | 6.7 | 4.1 |
| Completeness [%] | 92.0 | 80.9 |
| Redundancy | 6.1 * | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.1 * | 4 * | pH 8.1 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 2.15 (M) | |
| 3 | 1 | reservoir | ammonium sulfate | ||
| 4 | 1 | reservoir | potassium phosphate | 50 (mM) |
