1BSL

STRUCTURE OF ALKANAL MONOOXYGENASE BETA CHAIN

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1995-06
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	58.800, 62.000, 218.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.950
Rwork	0.188
RMSD bond length	0.016
RMSD bond angle	16.900 *
Data reduction software	XDS
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	1.950 *	1.950 *
Rmerge	0.041	0.180 *
Total number of observations	125700 *
Number of reflections	55442	6873 *
Completeness [%]	93.7	85.1 *
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-12.5 (mg/ml)
2	1	drop	phosphate	50 (mM)
3	1	drop	EDTA	0.5 (mM)
4	1	drop	dithiothreitol	0.25 (mM)
5	1	drop	ammonium sulfate	0.75 (M)
6	1	drop	sodium borate	125 (mM)
7	1	drop	succinic	25 (mM)
8	1	reservoir	ammonium sulfate	1.5 (M)