1BRY

BRYODIN TYPE I RIP

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-07-02
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	65.720, 77.563, 115.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.237
Rwork	0.237
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tcs
RMSD bond length	0.009
RMSD bond angle	23.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.043	0.109
Number of reflections	31074
<I/σ(I)>	25.9	8.7
Completeness [%]	89.1	68.7
Redundancy	3.6	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6		0.1M MES (PH 6.0), 23% MEPEG5K, 0.2M AMMONIUM SULFATE, 0.1M LICL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG5000