1BRX

BACTERIORHODOPSIN/LIPID COMPLEX

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-10
Detector	RIGAKU RAXIS IV
Spacegroup name	P 63
Unit cell lengths	60.850, 60.850, 108.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 2.300
R-factor	0.18 *
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1AT9 AND 2BRD
RMSD bond length	0.014
RMSD bond angle	1.653
Data reduction software	HKL
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.113 *	0.443 *
Total number of observations	250474 *
Number of reflections	9769
<I/σ(I)>	15.1	1.7
Completeness [%]	96.5	75.9
Redundancy	25.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6	20 *	pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	3.5 (mg/ml)
2	1	1	methylpentanediol	0.05 (%)
3	1	1	beta-octylglycopyranoside	1.2 (%)