1BRS

PROTEIN-PROTEIN RECOGNITION: CRYSTAL STRUCTURAL ANALYSIS OF A BARNASE-BARSTAR COMPLEX AT 2.0-A RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	207.250, 43.860, 84.710
Unit cell angles	90.00, 107.76, 90.00

Refinement procedure

Resolution	7.000 - 2.000
R-factor	0.173 *
RMSD bond length	0.014
RMSD bond angle	0.044
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.090 *
Total number of observations	142093 *
Number of reflections	43964 *
Completeness [%]	89.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		23 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20 (%)
2	1	reservoir	Tris-HCl	100 (mM)
3	1	reservoir	ammonium sulfate	100 (mM)
4	1	drop	protein	22 (mg/ml)