1BRR
X-RAY STRUCTURE OF THE BACTERIORHODOPSIN TRIMER/LIPID COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.520, 105.960, 80.190 |
| Unit cell angles | 90.00, 94.94, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.900 |
| R-factor | 0.256 |
| Rwork | 0.256 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2brd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 19.430 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.030 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.078 | 0.215 |
| Total number of observations | 48145 * | |
| Number of reflections | 18504 | |
| <I/σ(I)> | 10.2 | 5.7 |
| Completeness [%] | 82.3 | 43.6 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.2 | 4 * | SEE REFERENCE 2, pH 5.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9-11.5 (mg/ml) | |
| 2 | 1 | drop | beta-octylglucopyranoside | 0.25 (%(w/v)) | |
| 3 | 1 | drop | benzamidine | 2 (%(w/v)) | |
| 4 | 1 | drop | sodium phosphate | 250 (mM) | |
| 5 | 1 | drop | sodium phosphate | 1.5 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.8-2.3 (M) |
