1BRR

X-RAY STRUCTURE OF THE BACTERIORHODOPSIN TRIMER/LIPID COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-07-15
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	120.520, 105.960, 80.190
Unit cell angles	90.00, 94.94, 90.00

Refinement procedure

Resolution	10.000 - 2.900
R-factor	0.256
Rwork	0.256
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2brd
RMSD bond length	0.009
RMSD bond angle	19.430 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.030
High resolution limit [Å]	2.900	2.900
Rmerge	0.078	0.215
Total number of observations	48145 *
Number of reflections	18504
<I/σ(I)>	10.2	5.7
Completeness [%]	82.3	43.6
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.2	4 *	SEE REFERENCE 2, pH 5.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9-11.5 (mg/ml)
2	1	drop	beta-octylglucopyranoside	0.25 (%(w/v))
3	1	drop	benzamidine	2 (%(w/v))
4	1	drop	sodium phosphate	250 (mM)
5	1	drop	sodium phosphate	1.5 (M)
6	1	reservoir	ammonium sulfate	1.8-2.3 (M)