1BRP

CRYSTAL STRUCTURE OF THE TRIGONAL FORM OF HUMAN PLASMA RETINOL-BINDING PROTEIN AT 2.5 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	H 3
Unit cell lengths	104.000, 104.000, 74.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	9.000 * - 2.500
R-factor	0.176
Rwork	0.176
RMSD bond length	0.008
RMSD bond angle	2.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.500 *	2.500 *
Rmerge	0.096 *
Total number of observations	29524 *
Number of reflections	9828 *
Completeness [%]		76 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	6.8 *	4 *	referred to J.Mol.Biol. 163.679-681 1983 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	RBP	20 (mg/ml)
2	1	1	dithioerythritol	1 (mM)
3	1	1	cacodylate	20 (mM)
4	1	1	ammonium acetate	3 (M)
5	1	2		4.5 (M)
6	1	2	cacodylate	20 (mM)