1BRL

THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Detector technology	IMAGE PLATE
Collection date	1993-10
Detector	FUJI
Spacegroup name	P 21 21 21
Unit cell lengths	59.900, 112.700, 301.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.208
RMSD bond length	0.016
RMSD bond angle	20.193 *
Data reduction software	PURDUE ((OSC))
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.115
Total number of observations	290488 *
Number of reflections	74342 *
Completeness [%]	92.0 *
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	luciferase	20 (mg/ml)
2	1	reservoir	ammonium sulfate	1.4 (M)
3	1	reservoir		200 (mM)
4	1	reservoir	succinate	100 (mM)