1BRK

BARNASE MUTANT WITH ILE 96 REPLACED BY ALA

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 32
Unit cell lengths	59.050, 59.050, 81.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 2.000
R-factor	0.149
RMSD bond length	0.015
RMSD bond angle	0.047
Data reduction software	MOSFLM
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.079
Total number of observations	32633 *
Number of reflections	19313
Completeness [%]	89.8
Redundancy	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	23 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	reservoir	ammonium sulfate	45 (%sat)
3	1	reservoir	ammonium phosphate	3.0 (M)
4	1	reservoir	zinc sulfate	0.02 (M)