1BRJ

BARNASE MUTANT WITH ILE 88 REPLACED BY ALA

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 32
Unit cell lengths	58.840, 58.840, 81.610
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 2.000
R-factor	0.17
RMSD bond length	0.015
RMSD bond angle	0.048
Data reduction software	MOSFLM
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.092
Total number of observations	39885 *
Number of reflections	19521
Completeness [%]	91.6
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	23 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	reservoir	ammonium sulfate	45 (%sat)
3	1	reservoir	ammonium phosphate	3.0 (M)
4	1	reservoir	zinc sulfate	0.02 (M)