1BRE

IMMUNOGLOBULIN LIGHT CHAIN PROTEIN

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-01-20
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	82.290, 77.730, 82.200
Unit cell angles	90.00, 119.95, 90.00

Refinement procedure

Resolution	5.000 - 2.000
R-factor	0.218
Rwork	0.218
R-free	0.33700
RMSD bond length	0.013
RMSD bond angle	2.100
Data reduction software	R-AXIS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	71.200
High resolution limit [Å]	1.760
Rmerge	0.101
Number of reflections	42843
Completeness [%]	47.2
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	23 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.25-2.5 (M)
2	1	reservoir	citrate	100 (mM)