1BQG
THE STRUCTURE OF THE D-GLUCARATE DEHYDRATASE PROTEIN FROM PSEUDOMONAS PUTIDA
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 273 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-04-02 |
| Detector | SIEMENS |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 69.623, 108.786, 122.573 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| Rwork | 0.190 |
| Structure solution method | MIR |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.500 |
| Data reduction software | XDS |
| Data scaling software | XSCALIBRE |
| Phasing software | HEAVY |
| Refinement software | TNT (5D) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.300 * |
| Rmerge | 0.040 | 0.202 |
| Total number of observations | 72242 * | |
| Number of reflections | 28914 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 88.0 | 70.8 * |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7 | 22 * | pH 7 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10.4 (mg/ml) | |
| 2 | 1 | 1 | PEG1000 | 19 (%) | |
| 3 | 1 | 1 | sodium formate | 100 (mM) | |
| 4 | 1 | 1 | BTP | 100 (mM) |
