1BQ9
Rubredoxin (Formyl Methionine Mutant) from Pyrococcus Furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Temperature [K] | 123 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS X-1000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.029, 34.474, 43.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.200 |
R-factor | 0.137 |
R-free | 0.16600 |
Structure solution method | OTHER |
RMSD bond length | 0.022 |
RMSD bond angle | 0.022 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.400 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.060 | 0.166 |
Total number of observations | 87169 * | |
Number of reflections | 15400 | |
Completeness [%] | 97.5 | 83.8 |
Redundancy | 5.6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | used macroseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | |
3 | 1 | drop | 0.3 (M) | ||
4 | 1 | reservoir | sodium potassium phosphate | 3.6 (M) |