1BQ6
CHALCONE SYNTHASE FROM ALFALFA WITH COENZYME A
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 97.631, 97.631, 65.303 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.560 |
| R-factor | 0.1654 |
| R-free | 0.21160 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1bi5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.025 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.500 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 45366 | |
| <I/σ(I)> | 23.8 | 5.3 |
| Completeness [%] | 91.5 | 69.6 |
| Redundancy | 3.5 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 4 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.2-2.4 (M) | |
| 3 | 1 | reservoir | PIPES | 0.1 (M) |
