1BOW

MULTIDRUG-BINDING DOMAIN OF TRANSCRIPTION ACTIVATOR BMRR (APO FORM)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	AREA DETECTOR
Detector	ADSC
Spacegroup name	P 41 21 2
Unit cell lengths	76.300, 76.300, 96.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.202
R-free	0.27400 *
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	1.290
Data scaling software	LOSCALE
Phasing software	PHASES
Refinement software	TNT (5D)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.060 *
Total number of observations	65480 *
Number of reflections	8264
<I/σ(I)>	10.3	1.6
Completeness [%]	100.0	100
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		Zheleznova, E.E., (1997) Protein Sci., 6, 2465. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		4.3 (M)
2	1	reservoir	Tris-HCl	20 (mM)	pH8.5
3	1	reservoir		2 (mM)