1BOQ

PRO REGION C-TERMINUS: PROTEASE ACTIVE SITE INTERACTIONS ARE CRITICAL IN CATALYZING THE FOLDING OF ALPHA-LYTIC PROTEASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1992-07-25
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	66.200, 66.200, 80.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.100
R-factor	0.159
Rwork	0.159
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2alp
RMSD bond length	0.014
RMSD bond angle	2.890
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.200	2.200
Rmerge	0.059
Number of reflections	33422
Completeness [%]	99.0	99.1
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *		ambient temperature *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	lithium sulfate	1.3 (M)