1BOO
PVUII DNA METHYLTRANSFERASE (CYTOSINE-N4-SPECIFIC)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 289 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 0.98233,0.98211,0.92,1.072 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.800, 112.400, 59.300 |
Unit cell angles | 90.00, 109.20, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.28300 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 24.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.052 | 0.226 |
Total number of observations | 54787 * | |
Number of reflections | 14886 | |
<I/σ(I)> | 11.5 | 5.5 |
Completeness [%] | 99.7 | 97.5 |
Redundancy | 3.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.2 | 0.1 M HEPES PH 7.2, 0.2 M SODIUM ACETATE 20% POLYETHYLENE GLYCOL 400 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | HEPES | 0.1 (M) | |
2 | 1 | 1 | sodium acetate | 0.2 (M) | |
3 | 1 | 1 | PEG4000 | 20 (%(w/v)) |