1BOG
ANTI-P24 (HIV-1) FAB FRAGMENT CB41 COMPLEXED WITH AN EPITOPE-HOMOLOGOUS PEPTIDE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 104.430, 104.430, 295.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 91.000 - 2.600 |
| R-factor | 0.246 * |
| Rwork | 0.246 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2HFL |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.036 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 91.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.076 |
| Number of reflections | 30270 |
| Completeness [%] | 96.0 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Fab | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 3 | 1 | reservoir | 2-(N-morpholino)-propanesulfonic acid | 0.1 (M) | |
| 4 | 1 | reservoir | 0.02 (%) |
