1BO6
ESTROGEN SULFOTRANSFERASE WITH INACTIVE COFACTOR PAP AND VANADATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.292, 78.909, 80.362 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.24900 |
| Structure solution method | MR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 25.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.051 * | |
| Number of reflections | 35147 | |
| Completeness [%] | 96.3 | 90.5 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7 * | Kakuta, Y., (1998) Biochem. Pharmacol., 55, 313. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG6000 | 8 (%) | |
| 2 | 1 | 1 | potassium phosphate | 5 (mM) | pH7.2 |
| 3 | 1 | 1 | MES | 50 (mM) | pH6.0 |
| 4 | 1 | 1 | 25 (mM) |
