1BO1
PHOSPHATIDYLINOSITOL PHOSPHATE KINASE TYPE II BETA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-15 |
| Detector | MACSCIENCE |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 109.915, 182.403, 106.472 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 3.000 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.29900 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.455 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.052 * | |
| Total number of observations | 297963 * | |
| Number of reflections | 21621 | |
| <I/σ(I)> | 14.7 | 4.5 |
| Completeness [%] | 99.2 | 98.3 |
| Redundancy | 13.7 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 20 * | 100MM SODIUM CITRATE PH5.6 200MM MAGNESIUM ACETATE 100MM LITHIUM ACETATE 16% PEG (4000) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 2 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 3 | 1 | reservoir | lithium acetate | 100 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 13-17 (%) | |
| 5 | 1 | drop | protein | 13-17 (mg/ml) |
