1BNJ

BARNASE WILDTYPE STRUCTURE AT PH 9.0

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Detector technology	FILM
Spacegroup name	P 32
Unit cell lengths	59.530, 59.530, 81.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.159
RMSD bond length	0.020
RMSD bond angle	0.058
Data reduction software	MOSFLM
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.100
Rmerge	0.054
Number of reflections	20543
Completeness [%]	83.7
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	23 *	pH 9.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	45 (%sat)
2	1	reservoir	ammonium phosphate	3.0 (M)
3	1	reservoir	zinc sulphate	0.02 (M)
4	1	drop	protein	10-20 (mg/ml)