1BN8
BACILLUS SUBTILIS PECTATE LYASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.900, 89.000, 50.700 |
| Unit cell angles | 90.00, 108.93, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| Rwork | 0.179 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.019 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.069 |
| Number of reflections | 187670 |
| Completeness [%] | 100.0 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | Jenkins, J.A., (1992) J. Mol. Biol., 228, 1255. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 35-40 (%) | |
| 2 | 1 | reservoir | MOPS | 50 (mM) | |
| 3 | 1 | reservoir | ethanolamine | 50 (mM) |
