1BMT
HOW A PROTEIN BINDS B12: A 3.O ANGSTROM X-RAY STRUCTURE OF THE B12-BINDING DOMAINS OF METHIONINE SYNTHASE
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.700, 55.300, 103.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 23.500 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.000 * |
| Rmerge | 0.054 * |
| Number of reflections | 10417 * |
| Completeness [%] | 89.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG6000 | ||
| 2 | 1 | 1 | Tris-HCl | 50 (mM) |
