1BMO
BM-40, FS/EC DOMAIN PAIR
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.770, 56.350, 111.600 |
| Unit cell angles | 90.00, 104.38, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.100 |
| R-factor | 0.262 |
| Rwork | 0.262 |
| R-free | 0.32200 |
| Structure solution method | MOLECULAR REPLACEMENT AND 2-FOLD NCS AVERAGING |
| Starting model (for MR) | BM-40 EC DOMAIN (PDB ENTRY 1SRA) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | MAMA |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.086 | 0.267 |
| Total number of observations | 47563 * | |
| Number of reflections | 14328 | |
| <I/σ(I)> | 6.5 | 2.7 |
| Completeness [%] | 90.7 | 85.1 |
| Redundancy | 3.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | HANGING DROP VAPOUR DIFFUSION AT 20 DEG C: 10 MG/ML PROTEIN IN 10 MM TRIS PH 7.5 + 2 MM CACL2; 0.1 M HEPES PH 7.5, 0.15 M NA-ACETATE, 15% PEG4K, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4-5 (mg/ml) | |
| 2 | 1 | drop | Tris | 5 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | PEG4000 | 7 (%(w/v)) | |
| 5 | 1 | drop | sodium acetate | 0.075 (M) | |
| 6 | 1 | drop | Na HEPES | 0.05 (M) | |
| 7 | 1 | reservoir | PEG4000 | 14 (%(w/v)) | |
| 8 | 1 | reservoir | sodium acetate | 0.15 (M) | |
| 9 | 1 | reservoir | Na HEPES | 0.1 (M) |
