1BML

COMPLEX OF THE CATALYTIC DOMAIN OF HUMAN PLASMIN AND STREPTOKINASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1997-08-01
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	80.030, 125.050, 86.790
Unit cell angles	90.00, 105.41, 90.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.201
Rwork	0.201
R-free	0.29100
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	28.770 *
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	MLPHARE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.400	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.050 *	0.252 *
Number of reflections	33424
<I/σ(I)>	11.25	2.17
Completeness [%]	91.3	80.3 *
Redundancy	1.9	1.43

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8	20 *	pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	sodium citrate	1.0 (M)
3	1	reservoir	HEPES	0.2 (M)
4	1	reservoir	magnesium chloride	1 (mM)