1BM9
REPLICATION TERMINATOR PROTEIN FROM BACILLUS SUBTILIS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-06 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.191, 52.686, 70.583 |
| Unit cell angles | 90.00, 90.52, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.28100 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.700 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | PHASES |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.070 |
| Number of reflections | 16299 | |
| <I/σ(I)> | 7 | 2 |
| Completeness [%] | 84.9 | 70 |
| Redundancy | 2 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 | 20 (%(w/v)) | |
| 2 | 1 | reservoir | MES | 0.125 (M) |
