1BM3
IMMUNOGLOBULIN OPG2 FAB-PEPTIDE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-03-12 |
| Detector | SDMS |
| Spacegroup name | A 1 2 1 |
| Unit cell lengths | 74.110, 90.050, 79.110 |
| Unit cell angles | 90.00, 115.30, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| Rwork | 0.152 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1opg |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.062 |
| Data reduction software | NILSEN |
| Data scaling software | X-GEN ((HOWARD) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.044 |
| Number of reflections | 20334 |
| Completeness [%] | 78.0 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 22 * | 0.1M CACODYLATE, PH6.5, 0.2M CALCIUM ACETATE AND 16% PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein/peptide | 21 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 16 (%) | |
| 3 | 1 | reservoir | cacodylate | 0.1 (M) | |
| 4 | 1 | reservoir | calcium acetate | 0.2 (M) |
