1BM0

CRYSTAL STRUCTURE OF HUMAN SERUM ALBUMIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-05-14
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1
Unit cell lengths	59.360, 96.890, 59.240
Unit cell angles	91.04, 103.40, 74.94

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.207
Rwork	0.207
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HUMAN SERUM ALBUMIN (1AO6)
RMSD bond length	0.014
RMSD bond angle	21.100 *
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.470	2.500
High resolution limit [Å]	2.370	2.370
Rmerge	0.134	0.428
Number of reflections	36149
<I/σ(I)>	4.37	1.52
Completeness [%]	71.3	36 *
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	293 *	pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	150-255 (mg/ml)
2	1	drop	potassium phosphate	50 (mM)
3	1	drop	PEG400	30-38 (%(v/v))
4	1	drop	sodium azide	5 (mM)