1BLP

STRUCTURAL BASIS FOR THE INACTIVATION OF THE P54 MUTANT OF BETA-LACTAMASE FROM STAPHYLOCOCCUS AUREUS PC1

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	54.900, 96.000, 137.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.181
RMSD bond length	0.023
RMSD bond angle	0.042
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	4.180 *
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.084 *	0.267 *
Total number of observations	52480 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8 *		Herzberg, O., (1987) Science, 236, 694. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	78 (%sat)
2	1	1		0.3 (M)
3	1	1		100 (mM)