1BLN

ANTI-P-GLYCOPROTEIN FAB MRK-16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	AREA DETECTOR
Detector	SDMS
Spacegroup name	P 1 21 1
Unit cell lengths	54.490, 67.774, 117.186
Unit cell angles	90.00, 97.61, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.209
Rwork	0.209
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mcp
RMSD bond length	0.006
RMSD bond angle	27.200 *
Phasing software	X-PLOR
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.800	2.800
Rmerge	0.114 *	0.220 *
Total number of observations	56513 *
Number of reflections	18167	3111 *
<I/σ(I)>	9.7	2.1
Completeness [%]	86.0	75
Redundancy	4	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	4		Vasudevan, S., (1994) J. Mol. Biol., 241, 736. *