1BLL

X-RAY CRYSTALLOGRAPHIC DETERMINATION OF THE STRUCTURE OF BOVINE LENS LEUCINE AMINOPEPTIDASE COMPLEXED WITH AMASTATIN: FORMULATION OF A CATALYTIC MECHANISM FEATURING A GEM-DIOLATE TRANSITION STATE

Experimental procedure

Spacegroup name	P 63 2 2
Unit cell lengths	130.300, 130.300, 121.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 * - 2.400
R-factor	0.198
Rwork	0.198
RMSD bond length	0.015
RMSD bond angle	3.500
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.900 *
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.089 *	0.183 *
Number of reflections	23316 *	2019 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium phosphate	10 (mM)
3	1	reservoir	PEG4000	16 (%(w/v))	or 17%(w/v)