1BL8
POTASSIUM CHANNEL (KCSA) FROM STREPTOMYCES LIVIDANS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | PRINCETON 2K |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.780, 68.930, 112.040 |
| Unit cell angles | 90.00, 124.63, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.200 |
| R-factor | 0.28 |
| Rwork | 0.280 |
| R-free | 0.29000 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.730 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.086 | 0.286 * |
| Number of reflections | 12603 | |
| Completeness [%] | 93.3 | 66.6 |
| Redundancy | 6.1 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | 20 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | 200 (mM) | ||
| 6 | 1 | reservoir | HEPES | 100 (mM) | |
| 7 | 1 | reservoir | PEG400 | 48 (%) |
