1BKV
COLLAGEN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.090, 15.629, 39.715 |
| Unit cell angles | 90.00, 104.46, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IDEALIZED 7-FOLD TRIPLE HELIX |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.020 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.3) |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 * | 0.133 |
| Number of reflections | 4853 | |
| Completeness [%] | 96.0 * | 85 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 4 * | pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | 10.0 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 15 (%) | |
| 3 | 1 | drop | acetic acid | 0.05 (M) | |
| 4 | 1 | drop | Tris-HCl | 0.05 (M) | |
| 5 | 1 | drop | 0.1 (M) | ||
| 6 | 1 | reservoir | PEG4000 | 30 (%) | |
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 8 | 1 | reservoir | 0.2 (M) |
