1BKL

SELF-ASSOCIATED APO SRC SH2 DOMAIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	60.200, 71.900, 34.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.2
Rwork	0.200
R-free	0.30400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PROTEIN MODEL FROM 1SPS
RMSD bond length	0.013
RMSD bond angle	3.100
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.069	0.240
Number of reflections	6947
<I/σ(I)>	23.4	5
Completeness [%]	83.2	70.9
Redundancy	4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6		2M AMMONIUM SULFATE PH 6.6 , 25 MG/ML SRC SH2, SITTING DROPS, vapor diffusion - sitting drop