1BK9
PHOSPHOLIPASE A2 MODIFIED BY PBPB
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 290 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-04-05 |
| Detector | SIEMENS |
| Spacegroup name | P 61 |
| Unit cell lengths | 82.820, 82.820, 32.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.153 |
| Rwork | 0.153 |
| R-free | 0.22600 |
| Structure solution method | DIFFERENT FOURIER |
| Starting model (for MR) | ACIDIC PLA2 (PDB ENTRY 1PSJ) |
| RMSD bond length | 0.013 |
| RMSD bond angle | 22.400 * |
| Data reduction software | XENGEN (SOFTWARE) |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.010 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.062 | 0.034 |
| Number of reflections | 8600 | |
| <I/σ(I)> | 15.795 | 2.15 |
| Completeness [%] | 81.1 | 34.6 |
| Redundancy | 3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 18 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.5 (mg/ml) | |
| 2 | 1 | drop | 0.06 (M) | ||
| 3 | 1 | drop | 1,4-butanediol | 24.8 (%(v/v)) |
