1BK6

KARYOPHERIN ALPHA (YEAST) + SV40 T ANTIGEN NLS

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1997-12
Detector	BRANDEIS
Spacegroup name	P 21 21 21
Unit cell lengths	161.120, 75.750, 84.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.100 - 2.800
R-factor	0.256
Rwork	0.256
R-free	0.30700
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	UNLIGANDED KARYOPHERIN ALPHA
RMSD bond length	0.008
RMSD bond angle	16.870 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (0.2)
Refinement software	CNS (0.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.064 *	0.188 *
Total number of observations	270860 *
Number of reflections	22717
<I/σ(I)>	17.4	9.9
Completeness [%]	86.9	82.2
Redundancy	3.5	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	4 *	pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	18-20 (%(w/v))
2	1	reservoir	sodium acetate	200 (mM)
3	1	reservoir	HEPES	100 (mM)
4	1	reservoir		20 (mM)