1BK2
A-SPECTRIN SH3 DOMAIN D48G MUTANT
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.250, 42.220, 49.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.010 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.32500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shg |
| RMSD bond length | 0.015 |
| RMSD bond angle | 27.680 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.044 | 0.121 |
| Total number of observations | 47630 * | |
| Number of reflections | 4758 * | |
| Completeness [%] | 96.5 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.1 (M) | |
| 3 | 1 | reservoir | sodium citrate | 90 (mM) | |
| 4 | 1 | reservoir | bis-tris-propane | 90 (mM) | |
| 5 | 1 | reservoir | EDTA | 0.9 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 0.9 (mM) | |
| 7 | 1 | reservoir | sodium azide | 0.9 (mM) |
