1BJZ
TETRACYCLINE CHELATED MG2+-ION INITIATES HELIX UNWINDING FOR TET REPRESSOR INDUCTION
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.5 |
Synchrotron site | SRS |
Beamline | PX9.5 |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1996-08 |
Detector | MARRESEARCH |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 69.100, 69.100, 182.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.600 - 2.200 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2tct |
RMSD bond length | 0.007 |
RMSD bond angle | 18.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.600 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.083 * | 0.363 * |
Number of reflections | 10487 * | |
<I/σ(I)> | 8.2 | 4.1 |
Completeness [%] | 89.5 * | 93.1 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | 18 * | pH 8.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | TetR(D) | 0.4 (mM) | |
2 | 1 | drop | 200 (mM) | ||
3 | 1 | drop | Tris-HCl | 10 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 1 (M) | |
5 | 1 | reservoir | 200 (mM) | ||
6 | 1 | reservoir | Tris-HCl | 20 (mM) |