1BJQ
THE DOLICHOS BIFLORUS SEED LECTIN IN COMPLEX WITH ADENINE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.377, 116.135, 224.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.650 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fat |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.460 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.116 | 0.315 |
| Total number of observations | 162686 * | |
| Number of reflections | 57851 | |
| <I/σ(I)> | 12.3 | 6.5 |
| Completeness [%] | 92.4 | 69.1 |
| Redundancy | 2.8 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | Dao-Thi, M., (1998) Acta Crystallogr.,Sect.D, 54, 1446. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 8-12 (%(w/v)) | |
| 4 | 1 | reservoir | 20 (mM) |
