1BJO

THE STRUCTURE OF PHOSPHOSERINE AMINOTRANSFERASE FROM E. COLI IN COMPLEX WITH ALPHA-METHYL-L-GLUTAMATE

Experimental procedure

Source type	ROTATING ANODE
Source details	ELLIOTT GX-20
Temperature [K]	277
Detector technology	DIFFRACTOMETER
Collection date	1991-07
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 21 21 21
Unit cell lengths	68.860, 94.450, 131.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.800 - 2.800
R-factor	0.185
Rwork	0.185
R-free	0.25100
Structure solution method	DIFFERENCE FOURIER
RMSD bond length	0.010 *
RMSD bond angle	23.860 *
Data reduction software	MADNESS
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.800	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.094 *	0.402 *
Number of reflections	19908
<I/σ(I)>	9.8	2.8
Completeness [%]	91.8	68.4
Redundancy	2.7	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		4-7 *	pH7.2 in the drop and pH5.6 in the reservoir *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000
2	1	reservoir	sodium acetate