1BJF
CRYSTAL STRUCTURE OF RECOMBINANT BOVINE NEUROCALCIN DELTA AT 2.4 ANGSTROMS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.680, 93.350, 50.625 |
| Unit cell angles | 90.00, 98.29, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.30800 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 21.100 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR (3.84) |
| Refinement software | X-PLOR (3.84) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.490 | |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.073 | |
| Number of reflections | 12105 | |
| Completeness [%] | 84.3 | 54.2 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.1 | 20-25 * | pH 6.1 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-20 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 45-50 (%sat) | |
| 3 | 1 | reservoir | PIPES | 50 (mM) | |
| 4 | 1 | reservoir | 5 (mM) | ||
| 5 | 1 | reservoir | beta-mercaptoethanol | 0.5 (mM) |
