1BJ5
HUMAN SERUM ALBUMIN COMPLEXED WITH MYRISTIC ACID
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.5 |
Synchrotron site | SRS |
Beamline | PX9.5 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1997-04 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 189.180, 38.960, 96.400 |
Unit cell angles | 90.00, 105.31, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.2244 |
Rwork | 0.224 |
R-free | 0.27920 * |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 24.200 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.084 | 0.225 |
Number of reflections | 21836 | |
<I/σ(I)> | 5.4 | 3 |
Completeness [%] | 90.7 | 65 |
Redundancy | 4.8 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 | CRYSTALS WERE OBTAINED BY VAPOUR DIFFUSION USING THE SITTING DROP TECHNIQUE. EQUAL VOLUMES OF HSA-MYRISTIC ACID COMPLEX (100 MG/ML) WERE MIXED WITH A RESERVOIR SOLUTION CONSISTING OF 25-30% (W/ V) POLYETHYLENE GLYCOL 3350, 50MM POTASSIUM PHOSPHATE PH 7.5. CRYSTALS GREW SPONTANEOUSLY AS CLUSTERS OF RODS. STREAK AND MACROSEEDING WERE USED TO INCREASE CRYSTAL YIELD. CRYSTALS WERE HARVESTED INTO 31% (W/V) PEG 3350, 50MM POTASSIUM PHOSPHATE PH 7.5 CONTAINING 0.1 MM MYRISTIC ACID., vapor diffusion - sitting drop with streak and macroseeding |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 100 (mg/ml) | |
2 | 1 | reservoir | PEG3350 | 25-30 (%(w/v)) | |
3 | 1 | reservoir | potassium phosphate | 50 (mM) |