1BJ3
CRYSTAL STRUCTURE OF COAGULATION FACTOR IX-BINDING PROTEIN (IX-BP) FROM VENOM OF HABU SNAKE WITH A HETERODIMER OF C-TYPE LECTIN DOMAINS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-15 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.569, 56.794, 39.616 |
| Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.600 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.27100 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 25.300 * |
| Data reduction software | DENZO |
| Data scaling software | WEIS |
| Phasing software | PHASES (PROGRAM PACKAGE) |
| Refinement software | X-PLOR (2.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.102 | 0.145 |
| Total number of observations | 21947 * | |
| Number of reflections | 7253 | |
| <I/σ(I)> | 12 | 8.6 |
| Completeness [%] | 90.0 | 74 |
| Redundancy | 4.1 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 | 20 * | PROTEIN WAS CRYSTALLIZED BY MIXING WITH EQUAL VOLUME OF 60% AMMONIUM SULPHATE IN 20 MM TRI-HCL BUFFER AT PH 7.8 CONTAINING AND AT ROOM TEMPERATURE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 3 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 60 (%) | |
| 5 | 1 | reservoir | Tris-HCl |
